Semen

Analysis

At K9 Clinics semen testing services can be used with stud dogs semen collected from dogs around 12 months old and onwards. We check the overall Mobility of the sperm which is vital to ensure the sperm can move towards an egg. We also check the sperm count and morphology looking at the form and structure of the sperm cells.

Overview

of the service

Semen collection and evaluation is the best correlation available to determine if a dog has the potential to be fertile. A semen collection should be performed prior to breeding. Through this collection, the motility (movement) and morphology (appearance) of the semen can be evaluated.

Advantages

of doing so

The standard semen evaluation is evaluation of sperm morphology or cell shape. In order to evaluate morphology, the sperm need to be stained and evaluated at very high magnification or special microscopes can be used with unstained but dead sperm (phase contrast or differential interference contrast). There are numerous stains that can be used, but the 2 most common are eosin‐ nigrosin and Wright‐Giemsa. The eosin‐nigrosin stain would be the preferred one of the 2. This stain not only used for morphology but is also considered a vital stain. If it is applied correctly to the sperm, it can be used not only to evaluate sperm morphology but also viability. Sperm that are alive when the stain is mixed will allow no stain to diffuse across their membranes, so they appear white under the microscope; while sperm that are dead when the stain is mixed will allow a pink dye to cross the cell membrane, so they appear pink under the microscope.

Stages

A cup or tube fitted with a funnel is held over the tip of the penis to collect the ejaculate. Some breeders simply use a plastic bag held over the penis to collect semen. The first (prostatic, clear) fraction and the second (sperm-rich, cloudy) fraction should be collected.

Time

Anytime after 7 months in which we determine whether he is ready for a studding or not.

OUR

Expert team

K9 CLINICS are licensed to do Semen Analysis

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